Cast regolare

	Prima Stagione	Seconda Stagione	Terza Stagione
James Kirk	William Shatner	William Shatner	William Shatner
Spock	Leonard Nimoy	Leonard Nimoy	Leonard Nimoy
Leonard McCoy	DeForest Kelley	DeForest Kelley	DeForest Kelley
Montgomery Scott	James Doohan	James Doohan	James Doohan
Hikaru Sulu	George Takei	George Takei	George Takei
Nyota Uhura	Nichelle Nichols	Nichelle Nichols	Nichelle Nichols
Pavel Chekov		Walter Koenig	Walter Koenig